Coding

Part:BBa_K4259005:Design

Designed by: Jennifer Adami   Group: iGEM22_Leiden   (2022-10-07)


copA from E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 361
    Illegal PstI site found at 1531
    Illegal PstI site found at 1975
    Illegal PstI site found at 2122
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 361
    Illegal PstI site found at 1531
    Illegal PstI site found at 1975
    Illegal PstI site found at 2122
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 361
    Illegal PstI site found at 1531
    Illegal PstI site found at 1975
    Illegal PstI site found at 2122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 361
    Illegal PstI site found at 1531
    Illegal PstI site found at 1975
    Illegal PstI site found at 2122
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 2175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid was transformed into E. coli BL21


Source

The plasmid was obtained from the ASKA library

References